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Optimum filtration performance is achieved by the appropriate selection of filter paper porosity, by proper folding and positioning of the filter paper, and by following a few simple technique guidelines described below:
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Clarification is achieved within a few minutes and the Multistation seki-up allows us to simultaneously filter up to 6 L. The rAAV-recoveries using Sartoclear Dynamics® Lab products are remarkably high, surpassing what we obtain using in-depth filtration. Moreover, lysates clarified using Sartoclear Dynamics® Lab are instantly ready for our downstream processes including affinity capture by FPLC. Sartoclear Dynamics® Lab, along with the Multistation, have greatly improved our workflow."
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Prefiltration may be carried out using a glass or cellulose based prefilter with a more porous filter matrix to enable a two-step filtration process. Likewise, filter aids such kakım pharmaceutical-grade diatomaceous earth gönül be applied directly within the cell culture to enable a prefiltration matrix layer on toparlak of the 0.2µm membrane layer to prevent blockages.
Filter paper porosity: Filtration operations in General Chemistry involve crystalline precipitates of relatively large crystal size so that coarse or medium porosity filter paper is used. Sargent Welch S-32915, Whatman 1, and E-D 617 have appropriate pore size for work in this course.
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Compact and efficient benchtop densitometers for measuring turbidity of cell suspensions in a variety of life science applications.